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Therefore, other techniques must be used to obtain desired information, such as in vitro penetration and permeation studies. Concerns about the test's predictivity and reproducibility, plus animal welfare and political pressure in Europe, prompted a search for alternative test methods (Spielmann et al., ).

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Abnormal formation and organization of collagen network is commonly observed in many organ pathologies, but analytical techniques able to reveal the collagen biodistribution are still lacking.

Toxicol In Vitro. Mewes KR, Raus M, Bernd A, Zöller NN, Sättler A, Graf R. Elastin expression in a newly developed full-thickness skin equivalent.

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